Newsletter June 01

These newsletters are distributed at irregular intervals by Dag Lindgren. Email me if you want to be added or removed from the list or your email-address changed. I regret that insufficiencies in the interfacing between me and my computer sometimes causes problems in this (and other) processes, do not hesitate to focus my attention on that. An URL address to this Newsletter is at
http://www-genfys.slu.se/staff/dagl\Papers\Newsletters\Newsletter00to01\Newsletter_June01.htm
it may appear nicer formatted there.

 

NOVA 02

 

The 28:th Nordic PhD course in Plant Breeding has the theme  

QUANTITATIVE GENETICS AND EFFICIENT BREEDING

and takes place January 21-27, 2002 at Barnens Ö, Väddö, Stockholm, Sweden

 

The course website is

http://daglindgren.upsc.se/nova02/

I am arranging it, and will focus attention on some areas of relevance for forest tree improvement. Tim Mullin will be guest teacher. I think it is an extremely relevant activity for active forest geneticists with an interest for tree improvement as well as for active forest tree breeders. I strongly encourage those who subscribe to this email to participate (as “passive” if not “active”). I also ask you to encourage participation by others, who you believe will benefit from a better basis for understanding plant breeding activities. It is possible there is room in the schedule for letting “passive” play a more important role than in previous courses, if interested you can contact me. I have always seen these courses as an important part in as well the basic training as the maintenance of skill and contact net in the area. I participated in the first course as an active student and am now “crowning” my carrier by arranging the 28:th.

 

SLU’s field trials

There is a list of the field trials of the department of forest genetics and plant physiology at

http://www-genfys.slu.se/Project/field/field.htm

According to the departmental register our department has 107 “active” long time field trials. All are conifers. 79 were established before 1990, 28 1990-1995 and no after 1996.

There exists a Nordic register over long-term field trials, “noltfox”, to which SLU has reported centrally (based on departments reports and classifications). Under the subject field “Genetic diversity and breeding”, there are 300 trials at SLU, of which 40 were established 1990-1995 and 2 after 1995.

The distribution on current departments within SLU (on “active” field trials established later than 1989 with subject “genetic diversity and breeding”)

Forest Genetics and Plant Physiology:  23 trials; latest 1993
Forest Management: 4 trials; latest 1990
Silviculture: 15 trials; latest 1997
Forest Genetics: 0 trials; latest 1989

It may be noted that this trend does mainly reflect differences among departments, but seem to reflect an SLU trend. Forest genetics at SLU can certainly live some time without establishing field trials. There exist many trials in the field. And it may even be a reasonable “sharing of tasks” that future long term field activities are done by SkogForsk. But 

SLU´s  stated basic concept is :
To develop man's understanding and sustainable use of biological natural resources.
In my opinion this concept is not trustworthy if long term forest genetics field trials are left to SkogForsk. A governmental University ought to be more concerned with long term sustainability than the more applied industrial controlled “commercial” SkogForsk. I believe that the trend in recent establishment reflects a declining interest and competence to deal with old tests, which is a more serious matter.

 

Retirement

My former PhD student Eleonor White is retiring after 35 years with the Canadian Forest Service.  You get a feeling of the arrow of time when your former students start to retire….

 

 

Undergraduate Tree Breeding Course

Undergraduate Tree Breeding Course 2001 at Umeå will be taught Nov 5 - Dec 4 2001.

Five "skogsvetare" and 8 "foreign students" has registered.

Skogsvetare are rather few and not increasing compared with previous years, and experience says that half of them will not appear without ever reporting their planned absence.

Foreign students are more than 2000 (and their participation is according to experience more reliable).

Probably the reason for the increase from 2000 is that less alternatives in Uppsala are offered.

One may speculate around the future implications of that forest tree breeding has a better reputation among foreign than among Swedish students.

 

PLANTaktuellt

Skriften PLANTaktuellt lever och utvecklas. Den vänder sig i första hand

till plantskolister och skogsvårdsansvariga, men även till forskar- och

lärarkollegor i hela Norden. Eftersom den når den mest operativa målgruppen

när det gäller t.ex. skogsgenetiska spörsmål så kan det vara lämpligt att

 skogsgenetiker försöker förmedla sina resultat genom denna. Om man har

något handfast som man själv vill skriva en artikel om är det naturligtvis

välkommet. Ännu mer välkommet är dock tips, i stor och liten skala, som

kan förmedlas i artikel eller notisform. Skriv i så fall ett mail till

mats.hannerz@skogforsk.se eller Christer Nyström i Garpenberg (cny@du.se).

Den som är intresserad kan prenumerera på PLANTaktuellt (4 nr per år, 200

kr) genom att maila till Christer, eller gå in på SkogForsks hemsida

(www.skogforsk.se, se under forskningen, frö och plantor).

 

Gossip...
It is said that Gunnar Jansson will be half time paid by department of Forest Genetics and that some resource will be set aside to reconstruct the genetic lab at Umeå (probably it will close down in Sept, when the plant physiologists will leave the “Skogis” building in Umeå). It has been suggested that Gunnar Jansson and Bengt Andersson will look at the SLU genetic departments.

 

I repeat some paragraphs from the May01 newsletter with some specifications in italics (which also is used elsewhere to indicate late changes), as they may have been misunderstood.

Professor
Lärarförslagsnämnden has found Bengt Andersson qualified to professor in forest genetics, he will become adjunct professor at our department from July 1. That means that SkogForsk and SLU agrees he spends 20% of his time with SLU matters. It does not make that much difference, as we cooperate earlier also. He got a share of the teaching in forest genetics at Umeå. We interpret it as a visible sign of our good cooperation.

Degenerated plants?
We often talk about plants from stands as “natural” and plants from seed orchards as “improved”. I speculate if it might not be more justified to call plants from stands “degenerated” and plants from seed orchards as “natural”. Human has probably had a dysgenic influence on forests. At least in the north the forests were repeatedly creamed for the best trees. Therefore a single cycle of plus tree selection may just compensate for historical sins, and can be considered as a reconstruction of a more natural state. What do you think, is this reasonable? 
Another thought in the same line: Forestry wants a plant from each seed and a valuable tree from each plant, we are soon there, and this is very different from nature. That must also mean some negative selection. Part of the breeding effort is just compensating for that.

 

Clonal propagation

 

The thought struck me that clonal propagation can be done abroad. Seeds can be sent abroad for cloning. The cloned seeds or embryos can be further transported (returned). The transport can be done by ordinary “courier” mail without dangerous delays, large costs, large administrative difficulty or large risk for destruction because of unsuitable environments. Thus we are not dependent on being able to do somatic embryogenesis or artificial seeds in Sweden, but the “factory” can be somewhere else. Or there may be stronger reasons to – at some time in the future - consider developing and building a “seed cloning factory” in Sweden, as the potential market is much wider than Sweden.

 

I am much against the concept of patenting “genes”. I think it retards development (by complications and monopolitic tendencies), strengthens popular antipathy against forest tree breeding and repels capital from forest tree breeding (because of the long time involved and because there are not easy to market “commercial” profits in forest genetics). Patents connected to forest tree genes are among other things doubtful because 1) each clone constitutes a very small market, 2) very many clones must be dealt with both in forestry and in breeding, 3) we do not want to viewed at as the creators of “Frankenforests”; and much of these feelings have with focusing on large commercial companies focusing on controlling markets to do as much as with manipulating life, 4) a deep understanding of the conditions the different regional actors work under and their interactions is needed to develop a plant material (thus e.g. professional regional breeders, this means many and different people).

 

But now I try to argue that I see patents and large international companies as less doubtful in propagation than breeding, and I have fewer difficulties in seeing possible advantages (although I still think over emphasis on patenting and creating of monopoly can be is a doubtful thing). “Multiplication” of clones seldom functions without frictions. It is often small and inefficient systems supported by limited competence and which seldom are in the main focus of the operation, and not subject to real commercial pressure to do a good job. A “scientifically” based cooperation often focuses too much on the science and too little on professional service. The existence of a commercial service is likely to widen clonal propagation nearer to its optimal use, by making it more available for smaller and less dedicated operations.

 

For systems where a large international company, which does not claim any ownership of the genotypes or scientific partnership, makes just the clonal propagation, I see considerable advantages:

It can be used for mass-multiplication of good clones.

It can be used to get uniform material for clonal testing without own facilities or competence on a professional basis.

Note that clonal testing of the breeding material is often the most efficient way for long-term breeding or selection of seed orchard clones even if clonal forestry is not intended.

Clonal experiments have also a potential in science, which seldom was fully explored for the difficulty of getting uniform clonal material locally

Clones can be cryostored without own facilities or competence and thus preserved stable for later use

The system has the potential to be developed to a facility for assisting in the production of GMO material for testing

 

There seems to be possibilities for competition, reasonable time span between investment (contracts) and return of the investment. Thus there are possibilities for “the market” to force development. Patents in these areas are less likely to block development and more similar to “ordinary” patents. They are also (from the investors point of view) easier to defend.  Still this is much dependent on that the multiplication technique used in the local nurseries after the return of the cloned material can be simple, reliable, similar to current practices and inexpensive (thus that the cloned material is raised similar to seedlings).

 

At a recent visit Yousry El-Kassaby described such a system to me and us in Umeå, which I think sounded interesting...(I especially focused the attention on the system to Urban Eriksson and Jan Twetman), for further information, contact yelkassaby@cellfor.com

 

Why so few conifer GMO in field tests?

Conifers are important for commercial plantation forestry, e.g. in Sweden 99 per cent of all forest tree seedlings raised are conifers. GMO (Genetic Modified Organisms, GE) trees have become an important research target with the justification that this would create better trees, either directly through GMO-forestry or indirectly through a better basic understanding or supporting techniques (like flowering induction). Why does not research put more attention on conifers (even if that is more difficult), if the aim is applied rather than basic?

There is a recent article about transgenic trees tested in North America (Silvae Genetica 49:233-239). “Today only two field trials of transgenic trees have been planted in Canada” (two poplar trials). In Table 1 (confined trials in US) there are 3 conifers and 83 other tree species. I looked at
http://www.isb.vt.edu/cfdocs/fieldtests1.cfm
(which probably reflects the current situation) and found 15 pine and 1 spruce trial. (So the conifers may be on way up, but the 15 pine trials seem all to be the same species, institution and localisation.)

There is information about GMO “field trials” (environmental releases) in the European Union (EU) at
http://food.jrc.it/gmo/
This seems to include experiments with Scots pine and Norway spruce, but these experiments are not field trials, just outdoor pollinations with transgenic pollen. Thus where does not exist a single conifer field trial in the whole EU (among 1649 GMO trials of which 17 were with trees).

Why has the reluctance been so strong against establishing GMO trials with the most important and relevant conifer species?

 

I submitted the question above to FORESTGEN 010607. This initiated a row of contributions to the debate, which probably is the longest in the history of FORESTGEN (which, however, does not say much as forest geneticists are not much for this form of debate) and an additional discussion developed into comments to me, which was not visible on FORESTGEN. However, most replies were concerned with issues only slightly related to my question (why are there so few of the field trials with conifers?). I made a general end-comment as follows to FORESTGEN

My reaction

I submitted the question “Why so few conifer GMO in field tests?” to FORESTGEN June 6. Now I comment on the matter relating to the discussion that arouse and in general. 

GMO is now successfully and without evident negative effects on environment or human health used on more than 40 million hectare agricultural land (mainly US, Canada and Argentine). This is after only two decades of development, which is an extremely rapid implementation. Genetic engineering has a huge potential, and where goes very large research investments into GMO related matters. The technique must sooner or later spread to more countries, characters and crops.

Long rotation conifers seem to be among the last potential targets for GMO, the need of field evaluation is an important reason for that.  The long rotation time can be seen as a good reason not to invest in GMO for conifers (Friedman pointed out that longer rotation give less incitements for investment in intensive forestry). But science should still explore a potentially powerful practical option. Neither science explores the potential to understand how genetics actually works in the forests if no field trials are done.

It may be wrong that long rotation conifers will come very late (if ever). After accumulating some experience over rotation from short rotation conifers (e.g. radiata on NZ, c.f. reply by Walter), it may be concluded that benefits evident in tests over only a fraction of the rotation time (better establishment) are not likely to be counteracted by disadvantages appearing late. But even if such a decision probably will be found justified at some point in the future for some types of GMO, this point lies probably many decades and more long term field trials ahead.

We working with long term forestry should not be too worried about the time perspective, E.g. in Sweden investments in forest tree breeding started 1938 (in the never realised hope of commercialising triploids). After more than six decades this has lead to that more than half of the Swedish regeneration material originates from first generation seed orchards. So the long rotation time should not discourage scientists from getting started. But it seems to be in the World’s interest to encourage and follow efforts on places, where the conifer rotation time is shorter; and where conifer experiments are being established anyway (see reply by Walter and http://www.ermanz.govt.nz/search/srch_reg.htm).

Both public and private funds have enthusiastically supported research, which seemed to bring GMO forests closer. This in combination with GMOs attractive features for commercialisation (patents, “biotech”); the success in agriculture; and brave visions by some scientists, may have created an over trust in a fast and massive expansion of GMO into plantation forestry. But, at the same time, plantation trials were rather rare and mainly comprised model systems. This over trust in combination with strong emphases of characters in commercial agriculture, which “people” often perceive as ecologically doubtful (herbicide resistance and “home manufactured” pesticides), and its association to commercial interests and mechanisms which are not familiar to the forestry sector, have probably contributed to strong and premature demands from institutions like “green certification” (cf. reply by Burley); WWF (cf. reply by Bisoffi); and “Eco-terrorists” (cf. reply by Apedaile). Such “protests” also contribute to the high costs of establishing field trials (cf. reply by Walter). There may be reasons for a more relaxed attitude (cf contributions from Bisoffi, Walsh and Frankis).

Not tolerating GMO trees is a rather fresh decision in green certification, and I do not think it is trustworthy that recent standards and principles should exist there forever. There is much and frequent turbulence. A policy of never tolerating a single GMO tree, while tolerating 50 million hectares of agricultural equivalents, seems neither sustainable nor trustworthy (even if different standards for forestry and agriculture is common on other issues, think about fertilization, monoculture and herbicides etc.), especially when GMO in a future will spread more to crops more similar to forest trees. Field tests brings the GMO forest options closer in time, by making us more able to evaluate advantages and risks. Perhaps it will also contribute to a more relaxed attitude, if there exist a number of real GMO trees, which looks like real trees. It seems in line with WWF demands (cf. reply by Bisoffi) to establish field trials, as they are needed for risk-evaluation. Without field tests, the true potential can never be evaluated.

Scientists with GMO trials are justifiable worried for sabotage. Many GMO trials are not replicated. Conventional forest field trials are almost always replicated, and for Sweden I guess that the risk of “loosing” a site is something like 1/3. Why not use replications more often for GMO trials?  (That is not only because of the risk of sabotage.) 

It is technical difficult to make a conifer plant from a cell and GMO requires changes in propagation technique which are difficult to master (cf. response by Friedman). Conifer GMO trials seems actually to become more common (cf. response by Mullin & Walter), perhaps reflecting improvements in propagation techniques.  That “popular resistance” or high costs to an increasing degree prevent GMO field trials does not seem reflected in the statistics. These factors are not likely to hit harder against conifers than other tree species.

The lack of GMO conifer field trials may reflect a trend that Forest Science (Universities) does not trust in “genetic” field trials as a method to gain knowledge nowadays. For our department (I am a University Professor) we have responsibility for 107 active trials. All are with conifers. 79 were established before 1990; 28 1990-1995; and no after 1996. For our department the interest has certainly swung drastically towards “trial free non-conifer GMOs”. The trend seems similar at University level (Swedish University of Agricultural Sciences).

 

I was adviced an article in a past issue of

"Grain"  (which is the "Quarterly Newsletter of the Genetic Resources

Action International") titled "Can't See the Trees for the Wood".

http://www.grain.org/publications/set001-en.cfm

Among different official national GMO sites I found the NZ particular informative (they keep much documentation available)

http://www.ermanz.govt.nz/search/srch_reg.htm

One article among many:
The advantages and disadvantages of the application of genetic engineering to forest trees: a discussion
J.H. Mathews, and M.M. Campbell.
pp. 371-380.

 

http://www3.oup.co.uk/foresj/hdb/Volume_73/Issue_04/