Bengt Andersson och Dag Lindgren.
Dags föredragning till "Molekylära och biotekniska metoder" Uppsala 080514. Bengt och Dags utspel om dokument till Molekylära och biotekniska metoder
GMO
Student reactions
Dag Lindgren had a lecture/seminar ending the course part of the research
school 2006-12-01:
Immediate Applications of Biotech in Tree Breeding???? The intention was that this should at least partly be a
jump off for the continued work in group 2. Student reactions and suggestions were asked
for but few (if any) came. The research school students are unique in the
World! They have got a basic training on both classical and
molecular breeding with the goal to be able to communicate and combine both Worlds. They have got good funding
and been exposed to modern conferences and
leading scientists. If they do not know any immediate applications, no-one
knows and where are no! Dag L offered to send his lecture with e-mail to those who asked for it, but no-one
has asked (except Rosario, who was not present, but asked if I shared something).
Thus the interest to search for new
immediate applications seem low among the research school students.
Dag Lindgren suggests he develops a historical review
Historic review of earlier efforts with biotech in tree breeding
Breeding without Breeding
Some suggestions in the presentation for the whole auditorium
ForadlingssynpunkterMar07.ppt
Polycross with
forward selection and pollen parents decided by markers
Comment about SSRs for seed orchards and Breeding Without Breeding by Dag Lindgren February 2008.
I started molecular work as I thought that big advances had been done in the previous decades, since I worked with seed orchards with isozymes. Partly I was influenced by the results by a visiting Japanese postdoc. So I tried to work with SSRs with Xiao-Ru Wang and another Japanese Postdoc. I am rather disappointed by the progress made in two decades, and if I knew what I know now I would probably not have jumped into it, but it is too late to change and I can still hope for the best and we anyway do get results. The systems are more powerful than isozymes, but the progress seem to be a factor 4 and not a factor 20 in two decades (since 1988) for what can be done with a given resource, and you are more constrained by the technique which requires more fancy equipment and more specialised competence. David Hall should take up work but did not do it in two years because of waiting for someone else to get started, which indicates it is not as easy as thought.
Details about the work with "project" 2:
Email were send enquiring people for suggestions: Ove Nilsson, Rosario, David Neale .....
Bengt makes a suggestion of what to write before writing more....(not done yet)
From Neale: "thanks for your note and your interest in our work in trying to bring genomics into application in breeding. The workshop that Jill, Jen and I did in Antalya was really just a start, there is much more on the way. I'm working with a large group in the US, the names you know well, to put together an application to the USDA for a Conifer Translational Genomics Network. This will represent our best effort in moving the technology into breeding. We will share this with you when written by Feb 14. Furthermore, we would love to enlist your support and participation in this network. We believe strongly in the comparative genomic approach to things. For now, see
http://www.pinegenome.org/cgp/conifercap/ for a conceptual model of how we're thinking about things."--> David "The Swedish Breeding Program is going through a revision. Bengt Andersson and I have been asked to comment if where are new molecular methods, which should be considered for implementation in our (conifer) breeding program. We ask if you want to focus our attention on something? I screened through the Turkey workshop material, but I did not find an application in use in operative breeding or mature for immediate application. Or did I miss something? Dag"
From Ove Nilsson: "Inom 10 år: - MAS bör vara möjligt för en rad olika positiva egenskaper, men också för att välja bort oönskade egenskaper. Individidentifikation är definitivt möjligt - identifiera "far och mor". Vi har en mycket bättre kunskap om de gener som styr viktiga egenskaper och har också markörer för gynnsamma/ogynnsamma alleler. - Genotypning och mappning av egenskaper är ren rutin, går oerhört enkelt och är billigt. - Klontester av SE-plantor är pågående och de första (men inte slutliga) utvärderingarna har gjorts. Teknik för storskalig amplifiering är utvecklad, kanske även artificiella frön. - De första fälttesten med GMO poppel utvärderas.- Möjligen har de första fälttesten med GMO gran just anlagts.- Tekniker för att stimulera tidig blomning i fröplantager finns framtagen, samt tekniker för att rutinmässigt stimulera blomning hos juvenila träd." (Spara gärna till 2017).
Ross Whetten was suggested to contact by Rosario,
At a seminar
Allele Discovery of Economic Pine Traits (ADEPT) Workshop
Tim White and Dudley Huber Applications to Pine Breeding, I guess Huber could be contacted
Useful links:
http://www.igd.cornell.edu/MolecularMarkers/MolecularMarkersVol1.html
The "Neale-inspirated" "Dendrome" can be worth a visit while reviewing molecular genomic tools.
Last edit May 08